6CV7

Mouse Protocadherin-15 Extracellular Cadherin Domains 1 through 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	P 65 2 2
Unit cell lengths	114.444, 114.444, 172.011
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	99.111 - 1.692
R-factor	0.1699
Rwork	0.169
R-free	0.18760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4apx
RMSD bond length	0.062
RMSD bond angle	1.645
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.111	1.753
High resolution limit [Å]	1.692	1.692
Rmerge	0.115	1.970
Rmeas	0.119
Number of reflections	73884	6959
<I/σ(I)>	20.19	0.69
Completeness [%]	99.3	92.5
Redundancy	18.2	6.6
CC(1/2)	0.992	0.362

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	35% Ethylene glycol