6CUJ
Crystal structure of the C-terminal domain of neisserial heparin binding antigen (NHBA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2014-08-29 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.96862 |
| Spacegroup name | P 41 |
| Unit cell lengths | 77.030, 77.030, 57.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.000 - 1.805 |
| R-factor | 0.1935 |
| Rwork | 0.191 |
| R-free | 0.23830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2lfu |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.897 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.000 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmeas | 0.060 | |
| Number of reflections | 31127 | 5025 |
| <I/σ(I)> | 13 | 2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.6 | 4.7 |
| CC(1/2) | 0.990 | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 3,350, 0.2 M Sodium thiocyanate |
