6CU2

Structure of N-truncated R2-type pyocin tail fiber at 2.6 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2017-10-25
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 63 2 2
Unit cell lengths	59.865, 59.865, 398.049
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.805 - 2.580
R-factor	0.1974
Rwork	0.194
R-free	0.22870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ct8
RMSD bond length	0.009
RMSD bond angle	0.850
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.6.0)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.620
High resolution limit [Å]	2.580	7.000	2.580
Rmerge	0.237	0.128	0.783
Rmeas	0.240	0.130	0.825
Rpim	0.041	0.024	0.253
Total number of observations	433942
Number of reflections	14554	919	660
<I/σ(I)>	5.5
Completeness [%]	99.8	99.9	96.5
Redundancy	29.8	30.6	10
CC(1/2)		0.998	0.772

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	1:1 ratio of protein to precipitant (100mM HEPES pH 8.0, 1M Sodium acetate, 0.4mM LDAO)