6CTD

C-terminal domain truncation of the Mycobacterium tuberculosis Mechanosensitive Channel of Large Conductance MscL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.897380
Spacegroup name	P 1 21 1
Unit cell lengths	84.709, 110.313, 136.804
Unit cell angles	90.00, 91.20, 90.00

Refinement procedure

Resolution	22.829 - 5.800
R-factor	0.3714
Rwork	0.371
R-free	0.38730
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.177
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX ((1.12_2829: 2829))
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	6.480
High resolution limit [Å]	5.800	5.800
Rmerge	0.048	1.000
Rmeas	0.052	1.240
Number of reflections	7936
<I/σ(I)>	16.7	1.2
Completeness [%]	96.0	98.6
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277.15	0.1 M Tris HCl pH 8, 0.15 M CaCl2, 25% PEG 400, 0.25% Glycine pH 8