6CRO
CRYSTAL STRUCTURE OF LAMBDA-CRO BOUND TO A CONSENSUS OPERATOR AT 3.0 ANGSTROM RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Collection date | 1993-02-15 |
| Detector | SDMS |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 102.970, 102.970, 102.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| Rwork | 0.194 |
| Structure solution method | MIR, WITH SUBSEQUENT MOLECULAR REPLACEMENT |
| Starting model (for MR) | TRUNCATED VERSION OF THE PREVIOUS LOW-RESOLUTION COMPLEX STRUCTURE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 23.244 * |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | VERIFY ((RODERICK)) |
| Refinement software | TNT (5EB) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.072 * | |
| Total number of observations | 132786 * | |
| Number of reflections | 3758 | |
| Completeness [%] | 99.9 | |
| Redundancy | 17.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 293 | CRO PROTEIN WAS SUSPENDED IN 20MM SODIUM CACODYLATE PH6.9 THEN MIXED WITH A 30% EXCESS OF THE 19BP DNA FRAGMENT. THE COMPLEX WAS THEN MIXED WITH AN EQUAL VOLUME OF PRECIPITANT SOLUTION (70MM AMMONIUM SULFATE, 13% PEG3350) AND ALLOWED TO EQUILIBRATE VIA THE HANGING DROP METHOD AT ROOM TEMPERATURE. COCRYSTALS TYPICALLY TAKE 3-4 MONTHS TO APPEAR., vapor diffusion - hanging drop, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SODIUM CACODYLATE | ||
| 2 | 1 | 1 | (NH4)2SO4 | ||
| 3 | 1 | 1 | PEG 3350 | ||
| 4 | 1 | 2 | (NH4)2SO4 | ||
| 5 | 1 | 2 | PEG 3350 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein complex | 7.0 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 50-80 (mM) | |
| 3 | 1 | drop | PEG3350 | 11-14 (%) | |
| 4 | 1 | reservoir | ammonium sulfate | 100-160 (mM) | |
| 5 | 1 | reservoir | PEG3350 | 22-28 (%) |
