6CRD
INFLUENZA VIRUS NEURAMINIDASE SUBTYPE N9 (TERN) with tetrabrachion (TB) domain stalk
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.998, 142.326, 163.303 |
| Unit cell angles | 90.00, 91.48, 90.00 |
Refinement procedure
| Resolution | 40.040 - 2.570 |
| R-factor | 0.21264 |
| Rwork | 0.208 |
| R-free | 0.30564 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7nn9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.781 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.040 | 2.630 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.307 | 0.702 |
| Number of reflections | 108611 | 2590 |
| <I/σ(I)> | 2.7 | 1 |
| Completeness [%] | 77.6 | 70.3 |
| Redundancy | 2.7 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 100 mM HEPES buffer at pH7.0 with 10% PEG6000 |
