6CQO

Crystal Structure of mitochondrial single-stranded DNA binding proteins from S. cerevisiae (SeMet Labeled), Rim1 (Form2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2016-01-27
Detector	RDI CMOS_8M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	80.283, 101.576, 114.484
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.985 - 2.800
R-factor	0.2254
Rwork	0.223
R-free	0.27640
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.417
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AutoSol
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.985	2.873
High resolution limit [Å]	2.800	2.800
Rmerge	0.131	1.230
Rpim	0.051	0.505
Number of reflections	23661	2302
<I/σ(I)>	16
Completeness [%]	99.9
Redundancy	15.7
CC(1/2)	0.998	0.732

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	295	0.2 M MgCl2 and 20% (w/v) PEG 3350