6CQK

Crystal Structure of mitochondrial single-stranded DNA binding proteins from S. cerevisiae, Rim1 (Form1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2016-01-27
Detector	RDI CMOS_8M
Wavelength(s)	0.97845
Spacegroup name	C 2 2 21
Unit cell lengths	54.500, 153.140, 118.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.570 - 2.800
R-factor	0.24729
Rwork	0.246
R-free	0.27854
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6cqo
RMSD bond length	0.018
RMSD bond angle	1.846
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.570	2.873
High resolution limit [Å]	2.800	2.800
Rmerge	0.180	1.967
Rpim	0.050	0.527
Number of reflections	12631	1244
<I/σ(I)>	12
Completeness [%]	100.0
Redundancy	7.4
CC(1/2)	0.997	0.527

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	295	0.2 M MgCl2 and 20% (w/v) PEG 3350