6CQJ
Crystal structure of DR1 presenting the RQ13 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.984 |
| Spacegroup name | P 1 |
| Unit cell lengths | 68.684, 82.017, 83.050 |
| Unit cell angles | 61.56, 88.20, 86.47 |
Refinement procedure
| Resolution | 47.960 - 2.750 |
| R-factor | 0.2 |
| Rwork | 0.197 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mdo |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.170 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.960 | 2.860 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rpim | 0.076 | 0.417 |
| Number of reflections | 40739 | 4564 |
| <I/σ(I)> | 8.1 | |
| Completeness [%] | 98.6 | |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | HEPES pH 7.5, Na acetate, PEG 4000 |
