6CQG

YycF Effector Domain Structure without DNA bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-26
Detector	MAR CCD 165 mm
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	32.630, 46.490, 63.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.635 - 1.932
R-factor	0.1908
Rwork	0.184
R-free	0.23580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gxq
Data reduction software	XDS
Data scaling software	Aimless (0.5.28)
Phasing software	Auto-Rickshaw
Refinement software	PHENIX (1.13.2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.460	1.970
High resolution limit [Å]	1.930	1.930
Rmerge	0.073	0.571
Rmeas	0.077	0.634
Rpim	0.025	0.266
Number of reflections	7516	406
<I/σ(I)>	17
Completeness [%]	98.0	83.3
Redundancy	9.1	4.5
CC(1/2)	0.999	0.791

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	293	200mM ammonium formate, 20%(w/v) PEG 3350 pH 6.6