6CP9
Contact-dependent growth inhibition toxin - immunity protein complex from Klebsiella pneumoniae 342
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-09 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 102.643, 145.446, 84.281 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.724 - 2.550 |
| R-factor | 0.1833 |
| Rwork | 0.181 |
| R-free | 0.23220 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.965 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.144 | 2.270 |
| Number of reflections | 41852 | 2053 |
| <I/σ(I)> | 34.5 | 1.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 25.6 | 22.7 |
| CC(1/2) | 0.722 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | 0.1 M Na citrate pH 5.0, 8% PEG8000 |
