6CO9

Crystal structure of Rhodococcus jostii RHA1 IpdAB COCHEA-COA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-25
Detector	RAYONIX MX300HE
Wavelength(s)	1.54
Spacegroup name	P 43 21 2
Unit cell lengths	69.294, 69.294, 241.855
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.998 - 1.602
R-factor	0.1627
Rwork	0.161
R-free	0.18720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6co6
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.998	1.660
High resolution limit [Å]	1.602	1.602
Rmerge	0.044	0.406
Number of reflections	77704	6844
<I/σ(I)>	31.31
Completeness [%]	99.0
Redundancy	10.8
CC(1/2)		0.934

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.9 M ammonium sulfate, 0.2 M sodium potassium tartrate