6CNR
aducanumab apo Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.311, 64.126, 67.293 |
| Unit cell angles | 90.00, 95.85, 90.00 |
Refinement procedure
| Resolution | 46.500 - 2.090 |
| R-factor | 0.2122 |
| Rwork | 0.211 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lf3 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.605 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.160 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.057 | 0.486 |
| Number of reflections | 36105 | |
| <I/σ(I)> | 20.3 | |
| Completeness [%] | 99.3 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 297 | 19% PEG 3350 in 100 mM sodium acetate, and 300 mM lithium sulfate |
