Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 31 |
| Unit cell lengths | 40.500, 40.500, 202.896 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.344 - 2.151 |
| R-factor | 0.2065 |
| Rwork | 0.206 |
| R-free | 0.22880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | earlier version of PDB entry 6C2K unpublished DNA model |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.580 | 40.580 | 2.220 |
| High resolution limit [Å] | 2.150 | 8.870 | 2.150 |
| Rmerge | 0.049 | 0.024 | 0.864 |
| Rmeas | 0.055 | 0.027 | 0.951 |
| Rpim | 0.023 | 0.012 | 0.397 |
| Total number of observations | 114295 | ||
| Number of reflections | 20266 | 1732 | |
| <I/σ(I)> | 18.1 | ||
| Completeness [%] | 99.9 | 91 | 100 |
| Redundancy | 5.6 | 5 | 5.8 |
| CC(1/2) | 0.999 | 0.998 | 0.771 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 20% PEG-3350, 0.2M ammonium formate |
