6CN8

High-resolution structure of ClpC1-NTD binding to Rufomycin-I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-25
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.078100
Spacegroup name	P 41 3 2
Unit cell lengths	109.758, 109.758, 109.758
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.403 - 1.400
R-factor	0.1602
Rwork	0.159
R-free	0.18480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wdb
RMSD bond length	0.008
RMSD bond angle	1.112
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.403	1.430
High resolution limit [Å]	1.400	1.400
Rmeas	0.099	1.362
Number of reflections	44919	4391
<I/σ(I)>	58.7	2.5
Completeness [%]	99.9	99.73
Redundancy	38	24.1
CC(1/2)	0.999	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	292	0.2 M sodium/potassium phosphate, pH 6.2, 2.5 M sodium chloride