6CN3
Crystal structure of zebrafish Phosphatidylinositol-4-phosphate 5- kinase alpha isoform D236A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2014-10-24 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 89.327, 89.327, 156.655 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.948 - 3.351 |
| R-factor | 0.2324 |
| Rwork | 0.230 |
| R-free | 0.27170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4tz7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.227 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 3.470 |
| High resolution limit [Å] | 3.350 | 7.200 | 3.350 |
| Rmerge | 0.114 | 0.049 | 0.848 |
| Rmeas | 0.119 | 0.052 | 0.879 |
| Rpim | 0.032 | 0.015 | 0.231 |
| Total number of observations | 135819 | ||
| Number of reflections | 9691 | 1085 | 936 |
| <I/σ(I)> | 6 | ||
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 14 | 12.4 | 14.3 |
| CC(1/2) | 0.999 | 0.898 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294.15 | 10 mg/ml protein, mixed with 0.1 M MES pH 6.5, 12% PEG 20000. |
