6CMQ
Structure of human SHP2 without N-SH2 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.550, 56.200, 247.460 |
| Unit cell angles | 90.00, 93.99, 90.00 |
Refinement procedure
| Resolution | 82.290 - 2.900 |
| R-factor | 0.2504 |
| Rwork | 0.249 |
| R-free | 0.27640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dgp |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.445 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.290 | 3.010 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rpim | 0.517 | 0.765 |
| Number of reflections | 42597 | 4448 |
| <I/σ(I)> | 4.3 | |
| Completeness [%] | 99.2 | 99.4 |
| Redundancy | 4.6 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 100 mM ammonium formate, 22% PEG 3,350 and 1.5% xylitol |
