6CLY

Structure of human DNA polymerase beta complexed with 8-ClG as the template base in a 1-nucleotide gapped DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97648
Spacegroup name	P 1 21 1
Unit cell lengths	54.772, 79.668, 54.967
Unit cell angles	90.00, 105.60, 90.00

Refinement procedure

Resolution	19.917 - 2.186
R-factor	0.2213
Rwork	0.219
R-free	0.26780
RMSD bond length	0.004
RMSD bond angle	0.886
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.220
High resolution limit [Å]	2.180	2.180
Number of reflections	23552
<I/σ(I)>	20.8
Completeness [%]	100.0
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	14% to 23% PEG3400, and 350 mM sodium acetate in 50 mM imidazole (pH 7.5)