6CL5
Structure of P. aeruginosa R1 pyocin fiber PALES_06171 comprising C-terminal residues 323-701
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-04-30 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 144.504, 155.031, 200.481 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.690 - 2.324 |
| R-factor | 0.1706 |
| Rwork | 0.169 |
| R-free | 0.20560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6cl6 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.600 |
| Data reduction software | XDS (December 31, 2011) |
| Data scaling software | XDS (December 31, 2011) |
| Phasing software | PHASER (2.3.0) |
| Refinement software | PHENIX ((dev_3042: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.460 |
| High resolution limit [Å] | 2.324 | 2.324 |
| Rmeas | 0.105 | 0.614 |
| Number of reflections | 371962 | 58288 |
| <I/σ(I)> | 8.8 | 2.08 |
| Completeness [%] | 99.2 | 96.2 |
| Redundancy | 3.45 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 500 mM sodium malonate, 100 mM HEPES, pH 6.0 |
