6CJY

Crystal Structure of Mnk2-D228G in complex with Inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	P 32 2 1
Unit cell lengths	105.574, 105.574, 71.677
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 3.050
R-factor	0.24239
Rwork	0.239
R-free	0.30772
RMSD bond length	0.015
RMSD bond angle	1.782
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.700	3.120
High resolution limit [Å]	3.050	3.050
Number of reflections	8776
<I/σ(I)>	4
Completeness [%]	99.0
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		300.15	20-30% (w/v) polyacrylic acid 5100, 1-5% PEG 400, and 50 mM Hepes (pH 7.5)