6CJI

Candida albicans Hsp90 nucleotide binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	120
Detector technology	CCD
Collection date	2013-09-27
Detector	RIGAKU SATURN A200
Wavelength(s)	1.5418
Spacegroup name	P 43 2 2
Unit cell lengths	73.856, 73.856, 107.268
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.500 - 1.640
Rwork	0.173
R-free	0.18750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ah8
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.670
High resolution limit [Å]	1.640	1.640
Rmerge	0.069	0.800
Number of reflections	37157	1826
<I/σ(I)>	17.5	3.6
Completeness [%]	100.0	100
Redundancy	14.8	12.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298.15	100mM Hepes 7.5 2M ammonium sulfate 2% PEG 400