6CJ6
Structure of the poxvirus protein F9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-24 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.450, 75.080, 136.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.860 - 2.100 |
| R-factor | 0.2279 |
| Rwork | 0.226 |
| R-free | 0.26850 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.728 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.860 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 37485 | 1884 |
| <I/σ(I)> | 7.5 | |
| Completeness [%] | 93.8 | 67 |
| Redundancy | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Crystals were grown from a solution containing 25% PEG 8000, 10% ethanol, 1-5% cocktail mixture of low molecular alcohols, 30% sucrose at pH 8.5 buffered with Tris in the presence of 50mM NaCl. The protein concentration was 10mg/ml |
