6CI5
Crystal structure of the formyltransferase PseJ from Anoxybacillus kamchatkensis in complex with UDP-4,6-dideoxy-4-formamido-L-AltNAc and tetrahydrofolate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-26 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.420, 73.780, 41.800 |
| Unit cell angles | 90.00, 103.47, 90.00 |
Refinement procedure
| Resolution | 46.884 - 2.000 |
| R-factor | 0.220023852975 |
| Rwork | 0.218 |
| R-free | 0.23954 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ci2 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.540 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | phenix.refine (dev_2689) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.230 | 1.940 |
| High resolution limit [Å] | 1.700 | 1.900 |
| Rmerge | 0.042 | 0.984 |
| Number of reflections | 32206 | 1720 |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2 M ammonium sulfate, 0.1 M MES pH 6.5, 25.6% PEG5000 MME |
