6CHY

STRUCTURE OF CHEMOTAXIS PROTEIN CHEY

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	80
Detector technology	CCD
Spacegroup name	P 21 21 2
Unit cell lengths	95.780, 76.700, 32.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.330
R-factor	0.185 *
Rwork	0.185
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	MODIFIED WILD-TYPE CHEY
RMSD bond length	0.014
RMSD bond angle	0.048
Data reduction software	MADNES
Data scaling software	MADNES
Phasing software	MERLOT
Refinement software	PROFFT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.450
High resolution limit [Å]	2.300	2.330
Rmerge	0.051
Total number of observations	28137 *
Number of reflections	7861
Completeness [%]	73.0 *	43.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		4 *	18% PEG 3350 AND 0.2 M AMMONIUM SULFATE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	18 (%)
2	1	reservoir	ammonium sulfate	0.2 (M)