6CHX

High Resolution Crystal Structure of the Hemagglutinin H1 Head Domain of Influenza A virus Solomon Islands

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2018-02-09
Detector	RAYONIX MX-300
Wavelength(s)	0.97856
Spacegroup name	C 1 2 1
Unit cell lengths	159.315, 30.575, 78.718
Unit cell angles	90.00, 114.69, 90.00

Refinement procedure

Resolution	39.642 - 1.400
R-factor	0.1754
Rwork	0.174
R-free	0.21080
RMSD bond length	0.010
RMSD bond angle	1.036
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.730	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.048	0.499
Number of reflections	66743
<I/σ(I)>	14
Completeness [%]	96.7
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	0.1 M Tris-HCl pH 8.0, 20% PEG MME 2000