6CHV
Proteus vulgaris HigA antitoxin bound to DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-02-22 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.000 |
Spacegroup name | P 65 |
Unit cell lengths | 79.449, 79.449, 399.427 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.724 - 2.900 |
R-factor | 0.2265 |
Rwork | 0.225 |
R-free | 0.26010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mct |
Data reduction software | xia2 (0.5.474) |
Data scaling software | Aimless (0.6.2) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13-2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.410 | 40.410 | 3.060 |
High resolution limit [Å] | 2.900 | 9.170 | 2.900 |
Rmerge | 0.127 | 0.062 | 0.389 |
Rmeas | 0.132 | 0.065 | 0.404 |
Rpim | 0.035 | 0.019 | 0.109 |
Number of reflections | 76031 | 1020 | 4673 |
<I/σ(I)> | 11.1 | ||
Completeness [%] | 100.0 | 98.9 | 100 |
Redundancy | 13.8 | 12.8 | 13.8 |
CC(1/2) | 0.998 | 0.999 | 0.987 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M CaCl2 and 10-25% w/v PEG 3,350 |