6CHK

Crystal structure of LacI family transcriptional regulator from Lactobacillus casei, Target EFI-512911, with bound TRIS

Replaces:  4RK3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9790
Spacegroup name	H 3 2
Unit cell lengths	108.715, 108.715, 125.395
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.800
R-factor	0.1453
Rwork	0.144
R-free	0.19020
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.633
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELX
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.105
Number of reflections	26573
<I/σ(I)>	30.2	2.5
Completeness [%]	100.0	100
Redundancy	12.2	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	294	protein in 10 mM Bis-Tris, 500 mM sodium chloride, 10% glycerol, 5 mM DTT, TEV protease (1:100 ratio), reservoir: 0.17 M sodium acetate, 0.085 M Tris-HCl, pH 8.5, 25.5% w/v PEG4000, 15% w/v glycerol, cryoprotectant = reservoir