Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.85506 |
| Spacegroup name | P 1 |
| Unit cell lengths | 854.245, 855.315, 865.907 |
| Unit cell angles | 60.38, 60.43, 61.98 |
Refinement procedure
| Resolution | 129.000 - 3.800 |
| Rwork | 0.422 |
| R-free | 0.42700 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 129.000 | 3.900 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Number of reflections | 15015304 | |
| <I/σ(I)> | 2.8 | |
| Completeness [%] | 53.2 | |
| Redundancy | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100MM HEPES PH 7.0, 200MM CALCIUM ACETATE, 14% PEG550 MME, 15% GLYCEROL. |
