6CGS

mouse cadherin-7 EC1-2 adhesive fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4C
Synchrotron site	NSLS
Beamline	X4C
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-28
Detector	MAR CCD 165 mm
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	58.304, 82.431, 93.546
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.720
R-factor	0.162
Rwork	0.160
R-free	0.19900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6cgu
RMSD bond length	0.009
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (DEV_1839)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.780
High resolution limit [Å]	1.700	1.700
Rmerge	0.070	0.656
Number of reflections	48271	4704
<I/σ(I)>	27.33
Completeness [%]	99.8
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	21.5% (w/v) PEG 6000 0.1M MES pH 5.6 1M LiCl 30% glycerol (cryoprotectant)