6CGA
Structure of the PR-DUB complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 150.170, 65.630, 162.760 |
| Unit cell angles | 90.00, 114.35, 90.00 |
Refinement procedure
| Resolution | 43.672 - 3.500 |
| R-factor | 0.2465 |
| Rwork | 0.244 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uem |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.557 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.672 | 3.830 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 18300 | 4333 |
| <I/σ(I)> | 7.1 | 1.4 |
| Completeness [%] | 98.8 | 99.1 |
| Redundancy | 2.8 | 2.7 |
| CC(1/2) | 0.998 | 0.613 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8.5 | 291 | 0.1 M Bis-Tris propane pH 8.5, 0.05 M potassium iodide, 13% (w/v) PEG 3350 and 10% (v/v) MPD |
