6CG9

Crystal structure of Triosephosphate Isomerase from Zea mays (mexican corn)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-11-18
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	68.430, 49.400, 74.600
Unit cell angles	90.00, 92.23, 90.00

Refinement procedure

Resolution	30.628 - 1.800
R-factor	0.1476
Rwork	0.145
R-free	0.18810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jk2
RMSD bond length	0.007
RMSD bond angle	0.979
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.630	1.864
High resolution limit [Å]	1.800	1.800
Rmerge	0.046	0.200
Rmeas	0.065	0.282
Rpim	0.046	0.200
Number of reflections	46447	4594
<I/σ(I)>	8.84	3.08
Completeness [%]	99.9	99.54
Redundancy	2	1.9
CC(1/2)	0.995	0.903

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291.15	0.2 M Magnesium acetate tetrahydrate, 0.1 M Sodium cacadylate trihydrate pH: 6.5, 20% w/v Polyethylene glicol 8000