6CG7
mouse cadherin-22 EC1-2 adhesive fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.072 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 50.260, 45.077, 128.054 |
Unit cell angles | 90.00, 92.25, 90.00 |
Refinement procedure
Resolution | 19.927 - 2.705 |
R-factor | 0.2312 |
Rwork | 0.226 |
R-free | 0.27720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6cgu |
RMSD bond length | 0.002 |
RMSD bond angle | 0.525 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.046 | 0.496 |
Number of reflections | 15707 | 1480 |
<I/σ(I)> | 22.7 | |
Completeness [%] | 98.3 | |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295.15 | 17% v/v PEG6000, 0.1 M HEPES, pH 7.0, 5% ethylene glycol, cryoprotectant: 30% v/v ethylene glycol |