6CG7
mouse cadherin-22 EC1-2 adhesive fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.072 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 50.260, 45.077, 128.054 |
| Unit cell angles | 90.00, 92.25, 90.00 |
Refinement procedure
| Resolution | 19.927 - 2.705 |
| R-factor | 0.2312 |
| Rwork | 0.226 |
| R-free | 0.27720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6cgu |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.525 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.046 | 0.496 |
| Number of reflections | 15707 | 1480 |
| <I/σ(I)> | 22.7 | |
| Completeness [%] | 98.3 | |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295.15 | 17% v/v PEG6000, 0.1 M HEPES, pH 7.0, 5% ethylene glycol, cryoprotectant: 30% v/v ethylene glycol |
