6CG6
mouse cadherin-10 EC1-2 adhesive fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-18 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 87.524, 87.524, 67.758 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.735 - 2.707 |
| R-factor | 0.2401 |
| Rwork | 0.238 |
| R-free | 0.28330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6cgu |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.502 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.123 | 1.041 |
| Number of reflections | 7545 | 732 |
| <I/σ(I)> | 11.7 | |
| Completeness [%] | 99.9 | |
| Redundancy | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295.15 | 6% v/v 2-propanol, 0.1 M MES, pH 6.0, 0.2 M calcium acetate, cryoprotectant: 30% v/v ethylene glycol |
