6CFU

Structure of Human alpha-Phosphomannomutase 1 containing mutations R180K and R183K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-08-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	P 43 21 2
Unit cell lengths	50.650, 50.650, 213.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.280 - 2.244
R-factor	0.2223
Rwork	0.217
R-free	0.27390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fuc
RMSD bond length	0.008
RMSD bond angle	1.134
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.280	2.320
High resolution limit [Å]	2.240	2.240
Number of reflections	13960
<I/σ(I)>	9.62
Completeness [%]	98.4
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	20% polyethylene glycol 3350, 0.15 M DL-malate, pH 7.0, 50 mM MgCl2, and 8 mM beta-mercaptoethanol