6CF0
Sperm Whale Myoglobin H64V Mutant with Nitrite
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-17 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 6 |
| Unit cell lengths | 90.150, 90.150, 45.324 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 78.070 - 1.640 |
| R-factor | 0.16669 |
| Rwork | 0.165 |
| R-free | 0.19325 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2mgj |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.142 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.070 | 1.684 |
| High resolution limit [Å] | 1.641 | 1.641 |
| Rmeas | 0.120 | |
| Rpim | 0.054 | |
| Number of reflections | 20847 | |
| <I/σ(I)> | 5 | |
| Completeness [%] | 100.0 | |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 2.64 M ammonium sulfate, 100 mM Tris-Cl, 1 mM EDTA |
