6CEQ

The Aer2 Receptor from Vibrio cholerae is a Dual PAS-Heme Oxygen Sensor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-04
Detector	ADSC QUANTUM 210
Wavelength(s)	0.62790
Spacegroup name	P 32 2 1
Unit cell lengths	62.512, 62.512, 157.024
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.165 - 1.670
R-factor	0.2046
Rwork	0.203
R-free	0.23600
RMSD bond length	0.007
RMSD bond angle	1.120
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.620
High resolution limit [Å]	1.590	4.320	1.590
Rmerge	0.135	0.063	1.617
Rmeas	0.144	0.068	2.018
Rpim	0.049	0.023	1.184
Number of reflections	47018	2637	1345
<I/σ(I)>	7.3
Completeness [%]	96.7	98.7	56.6
Redundancy	8.6	8.5	2
CC(1/2)		0.991	0.196

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	290	2.6 M ammonium sulfate, 0.1 M citric acid, pH 5.5