6CDA

Crystal structure of L34A CzrA in the Zn(II)bound state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2017-05-17
Detector	RDI CMOS_8M
Wavelength(s)	1.00003
Spacegroup name	P 41 21 2
Unit cell lengths	31.987, 31.987, 183.392
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.848 - 2.000
R-factor	0.2008
Rwork	0.199
R-free	0.24240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1r1u
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.850	2.072
High resolution limit [Å]	2.000	2.000
Rmeas	0.101	1.112
Rpim	0.028	0.304
Number of reflections	6867
<I/σ(I)>	17.6	2.5
Completeness [%]	96.4	100
Redundancy	12.4	13
CC(1/2)	0.999	0.878

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	0.1 M bis-tris propane (pH 9) and polyethylene glycol 550 20-22%