6CDA
Crystal structure of L34A CzrA in the Zn(II)bound state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2017-05-17 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 1.00003 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 31.987, 31.987, 183.392 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.848 - 2.000 |
| R-factor | 0.2008 |
| Rwork | 0.199 |
| R-free | 0.24240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r1u |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.850 | 2.072 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmeas | 0.101 | 1.112 |
| Rpim | 0.028 | 0.304 |
| Number of reflections | 6867 | |
| <I/σ(I)> | 17.6 | 2.5 |
| Completeness [%] | 96.4 | 100 |
| Redundancy | 12.4 | 13 |
| CC(1/2) | 0.999 | 0.878 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 0.1 M bis-tris propane (pH 9) and polyethylene glycol 550 20-22% |
