6CCA
Crystal structure of DszA carbon methyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-10-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.457, 76.549, 57.186 |
| Unit cell angles | 90.00, 90.51, 90.00 |
Refinement procedure
| Resolution | 57.180 - 1.750 |
| R-factor | 0.18013 |
| Rwork | 0.178 |
| R-free | 0.21065 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.829 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.180 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.066 | 0.558 |
| Number of reflections | 48867 | |
| <I/σ(I)> | 19.1 | |
| Completeness [%] | 99.5 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1M Bicine pH 8.5, 20% PEG 6000 |
