6CC1
Crystal structure of ykoY-alx riboswitch chimera bound to cadmium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 31 |
| Unit cell lengths | 73.089, 73.089, 117.082 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.978 - 2.540 |
| R-factor | 0.2288 |
| Rwork | 0.222 |
| R-free | 0.28100 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.666 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.096 | 1.000 |
| Rmeas | 0.112 | |
| Rpim | 0.056 | |
| Number of reflections | 29350 | 1358 |
| <I/σ(I)> | 33.99 | |
| Completeness [%] | 99.0 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 40 mM Sodium Cacodylate, 80mM Sodium Chloride, 20 mM Barium Chloride, 5 mM Cadium Chloride, 12mM sprimine tetrahydrochloride, 12% 2-methyl-2,4-pentanediol |
