6CBY

Crystal structure of human SET and MYND Domain Containing protein 2 with MTF9975

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97891
Spacegroup name	P 1 21 1
Unit cell lengths	57.819, 117.579, 65.013
Unit cell angles	90.00, 92.42, 90.00

Refinement procedure

Resolution	43.590 - 2.550
R-factor	0.201
Rwork	0.199
R-free	0.25800
RMSD bond length	0.010
RMSD bond angle	1.090
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.590
High resolution limit [Å]	2.550	6.920	2.550
Rmerge	0.204	0.106	0.934
Rmeas	0.227	0.118	1.041
Rpim	0.098	0.052	0.453
Total number of observations	151491
Number of reflections	28751	1480	1426
<I/σ(I)>	4
Completeness [%]	98.9	97.8	99.2
Redundancy	5.3	5.3	5.1
CC(1/2)		0.984	0.489

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	20% PEG 3350, 0.2 M NH4SO4