6CBS

DnaG Primase C-terminal domain complex with SSB C-terminal peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.953715
Spacegroup name	P 21 21 2
Unit cell lengths	69.895, 115.202, 45.817
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.574 - 1.850
R-factor	0.194
Rwork	0.192
R-free	0.22440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6cbt
RMSD bond length	0.004
RMSD bond angle	0.641
Data reduction software	iMOSFLM (6.2.6)
Data scaling software	SCALA (3.2.15)
Phasing software	MOLREP (9.4.09)
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	1.950
High resolution limit [Å]	1.850	1.850
Rmerge	0.072	0.463
Rmeas	0.078	0.503
Rpim	0.030	0.193
Number of reflections	30129	4449
<I/σ(I)>	15.3	3.8
Completeness [%]	93.1	96.2
Redundancy	6.2	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	294	7.5% w/v PEG3000, 15 mM zinc acetate, 100 mM sodium acetate, pH 4.6