6CBS
DnaG Primase C-terminal domain complex with SSB C-terminal peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.953715 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 69.895, 115.202, 45.817 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.574 - 1.850 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.22440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6cbt |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.641 |
| Data reduction software | iMOSFLM (6.2.6) |
| Data scaling software | SCALA (3.2.15) |
| Phasing software | MOLREP (9.4.09) |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.072 | 0.463 |
| Rmeas | 0.078 | 0.503 |
| Rpim | 0.030 | 0.193 |
| Number of reflections | 30129 | 4449 |
| <I/σ(I)> | 15.3 | 3.8 |
| Completeness [%] | 93.1 | 96.2 |
| Redundancy | 6.2 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 294 | 7.5% w/v PEG3000, 15 mM zinc acetate, 100 mM sodium acetate, pH 4.6 |
