6CBJ

Crystal Structure of DH270.3 Fab in complex with Man9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-27
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97925
Spacegroup name	I 41
Unit cell lengths	128.087, 128.087, 91.875
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.043 - 2.850
R-factor	0.2052
Rwork	0.204
R-free	0.23090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tpl
RMSD bond length	0.006
RMSD bond angle	0.950
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.043	2.950
High resolution limit [Å]	2.850	2.850
Number of reflections	17375	9657
<I/σ(I)>	11.66
Completeness [%]	99.6
Redundancy	3.8
CC(1/2)	0.992	0.422

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	1 M sodium/potassium phosphate, pH 5.6