6CBC
Crystal structure of an N-terminal fragment of Vps13.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-17 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.933, 85.984, 71.027 |
| Unit cell angles | 90.00, 93.67, 90.00 |
Refinement procedure
| Resolution | 48.543 - 3.000 |
| R-factor | 0.2317 |
| Rwork | 0.230 |
| R-free | 0.26420 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.452 |
| Data reduction software | XDS |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.600 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 28582 | |
| <I/σ(I)> | 21.9 | |
| Completeness [%] | 99.9 | |
| Redundancy | 20.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 310 | 100 mM Bis-Tris pH 6.5-6.7 and 34-38% 7 15/4 Pentaerythritol ethoxylate (Hampton). Drops were equilibrated against well solution containing 1 M NaCl |
