6CB2
Crystal structure of Escherichia coli UppP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-11 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 109.880, 146.080, 40.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.040 - 2.000 |
| R-factor | 0.2204 |
| Rwork | 0.219 |
| R-free | 0.25350 |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 73.150 | 73.150 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Rmerge | 0.108 | 0.026 | 1.580 |
| Rmeas | 0.118 | 0.030 | 1.804 |
| Rpim | 0.048 | 0.013 | 0.844 |
| Total number of observations | 124700 | 1601 | 4464 |
| Number of reflections | 21698 | 304 | 1156 |
| <I/σ(I)> | 8.1 | 24.8 | 1.1 |
| Completeness [%] | 96.7 | 99.6 | 73.1 |
| Redundancy | 5.7 | 5.3 | 3.9 |
| CC(1/2) | 0.999 | 1.000 | 0.397 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 45-50% PEG 200, 0-150 mM NaCl, 200-400 mM LiSO4 |
