6CAY
Crystal structure of the first StART-like domain of Ysp2p/Lam2p in its apo and ergosterol-bound state.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.594, 63.562, 77.540 |
| Unit cell angles | 90.00, 103.58, 90.00 |
Refinement procedure
| Resolution | 75.373 - 2.000 |
| R-factor | 0.1632 |
| Rwork | 0.161 |
| R-free | 0.20080 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.936 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.400 | 1.960 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Number of reflections | 42580 | |
| <I/σ(I)> | 30.4 | |
| Completeness [%] | 94.4 | |
| Redundancy | 20.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.9 | 295.15 | 32.5% PEG 3350, 0.1 M sodium acetate pH 4.9, 0.2 M ammonium acetate |
