6CAR

Serial Femtosecond X-ray Crystal Structure of 30S ribosomal subunit from Thermus thermophilus in complex with Sisomicin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	402.600, 402.600, 175.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.262 - 3.400
R-factor	0.2219
Rwork	0.222
R-free	0.26610
RMSD bond length	0.006
RMSD bond angle	0.995
Data reduction software	MOSFLM
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.262	39.262
High resolution limit [Å]	3.400	3.400
Number of reflections	196314
<I/σ(I)>	2.99
Completeness [%]	100.0
Redundancy	182.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	298	17% MPD