6CAQ

Crystal Structure of 30S ribosomal subunit from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	400.970, 400.970, 175.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.908 - 3.400
R-factor	0.1899
Rwork	0.190
R-free	0.21990
RMSD bond length	0.004
RMSD bond angle	0.839
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.908	39.908	3.210
High resolution limit [Å]	3.130	13.980	3.130
Rmerge	2.439	0.093	14.212
Rmeas	2.455	0.094	14.369
Total number of observations	18868280
Number of reflections	242707	2893	12722
<I/σ(I)>	8.45	59.44	0.33
Completeness [%]	97.4	93.3	69.7
Redundancy	77.741	66.248	41.749
CC(1/2)	0.865	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	298	17% MPD