6C95
The Human NatA (Naa10/Naa15) amino-terminal acetyltransferase complex bound to HYPK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-24 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 63 |
| Unit cell lengths | 182.320, 182.320, 86.070 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.040 - 3.150 |
| R-factor | 0.223 |
| Rwork | 0.221 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.944 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1-2575)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.043 | 3.270 |
| High resolution limit [Å] | 3.152 | 3.150 |
| Rmerge | 0.013 | 0.148 |
| Number of reflections | 27025 | |
| <I/σ(I)> | 11.43 | 1.2 |
| Completeness [%] | 95.3 | 96.1 |
| Redundancy | 4.84 | 4.91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 19.5% PEG3350, 11% Tascimate, pH 6.5 |
