6C8O

RNA-activated 2-AIpG monomer, 3h soaking

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	99
Detector technology	CCD
Collection date	2016-05-19
Detector	MAR CCD 130 mm
Wavelength(s)	1
Spacegroup name	P 3 2 1
Unit cell lengths	46.557, 46.557, 83.343
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.850
R-factor	0.23384
Rwork	0.231
R-free	0.28186
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dhc
RMSD bond length	0.024
RMSD bond angle	2.783
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.920
High resolution limit [Å]	1.850	1.850
Rmerge	0.091	0.539
Rmeas	0.097	0.587
Rpim	0.033	0.225
Number of reflections	9369	888
<I/σ(I)>	21.91	2.38
Completeness [%]	99.7	97.7
Redundancy	8.6	5.5
CC(1/2)	0.957	0.916

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	10% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.040 M Sodium cacodylate trihydrate pH 7.0, 0.012 M Spermine tetrahydrochloride, 0.080 M Sodium chloride, 0.020 M Magnesium chloride