6C8N
RNA-activated 2-AIpG monomer complex, 2h soaking
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 99 |
| Detector technology | CCD |
| Collection date | 2016-05-19 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 46.975, 46.975, 83.097 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.24923 |
| Rwork | 0.246 |
| R-free | 0.30635 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dhc |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.660 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.079 | 0.567 |
| Rmeas | 0.083 | 0.617 |
| Rpim | 0.027 | 0.236 |
| Number of reflections | 8006 | 402 |
| <I/σ(I)> | 24.5 | 1.35 |
| Completeness [%] | 91.6 | 47.2 |
| Redundancy | 9 | 5.1 |
| CC(1/2) | 0.966 | 0.868 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 10% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.040 M Sodium cacodylate trihydrate pH 7.0, 0.012 M Spermine tetrahydrochloride, 0.080 M Sodium chloride, 0.020 M Magnesium chloride |
